Full-length CDNA Clonging Of Trichomonas Vaginalis Virus

Trichomonas vaginalis, which parasitizes in urogenital system, is one kind of flagellates causing vaginal inflammation. At present, the characterization of T.vaginalis has not been understood completely and the discovery of Trichomonas vaginalis virus(TVV) played a pivotal role for T.vaginalis study in molecular biology. Several papers suggested that there were different kinds of dsRNA viruses in T.vaginalis. The T.vaginalis with virus was isolated from patients in Changchun and culture was carried out in vitro, the full-length cDNA of Trichomonas vaginalis virus was sequenced and constructed.T.vaginalis cultured in vitro T.vaginalis isolated from Changchun patients were cultured successfully in liver medium in vitro. The T.vaginalis strain with virus which had a 5.5kb extra band except genomic DNA was selected by 1% agarose gel electrophoresis analysis on total nucleic acids extracted from T. vaginalis. The T.vaginalis strain selected was cultured under axenic condition. The body configuration of paradises was observed by light and electron microscope. Growth state was very well in ox liver medium (pH=5.54) and the vital force didn't decrease after 50 generation. The strain freezed in 10% DMSO was cultured continuously after recovery and the...