Establishment And Characterization Of A Mesenchymal Chondrosarcoma Cell Line

Mesenchymal chondrosarcoma (MCS), first described in 1959 by Lichtenstein & Bernstein, is a very rare malignant parenchyma tumour. Most commonly, the causative tumors are of vascular, mesenchymal, or fibrous origin. Also, some scholars believe that they can occur at any location containing mesenchymal cells. Pathologic examination of the tumor revealed that it is characterized by a bimorphic histologic pattern: coexistence of nests of well-defined hyaline cartilaginous tissue within a proliferation of primitive hemangiopericytomalike or fibroblastlike mesenchymal cells. So far, the most documents of MCS are case reports, the most of the other studies are aimed at immunohistochemistry and ultramicrostructure of the tumor. And there is no report on establishment of implant model in vivo.We acquired a new cell line in the experiment by chance. By studying its proliferation, chromosome, ultromicrostructure and other bionomics by immunohistochemistry/immunocytochemistry, transfecting EGFP and transplanting in nude mice in vivo, it is discovered that the feature of the cell line is the same as MCS, so we name it MS(malignant sarcoma) 0812.It is shown in the experimental results that MS0812 cells keep a thriving growth in vitro, the time span of multiplying is about 20 h and it is not seen any inhibiting evidence of its growth. The MS0812 cells can not form any plaque in soft agar medium. The chromosome of the cell is anorthoploid. Microvilli was detected on the cell surface, lipid droplet and glycogen granule were detected in the plasma by TEM. The cells express troponin I and PAS.The results of immunocytochemistry demonstrate that...