Synthesis And Characterization Of Adsorbents For Removing Bilirubin

In order to improve the adsorption capacity for bilirubin in albumin solution and human plasma, Sepharose CL-4B and cellulose are selected as supports to prepare adsorbents, on which several functional groups are immobilized based on hydrogen bonds, electrostatic forces and hydrophobic interaction. The relativity between logP of functional groups and adsorption capacity for bilirubin is obtained by ChemOffice. The adsorption mechanism and effects of environments are researched as well.Experimental results show: (1) There are relativities between logP of functional groups and adsorption capacity in some extent. The logP of adsorbent based on Sepharose CL-4B as support and coupled the butylamine as terminal functional group is 0.8978, and it is close to that of bilirubin which is 1.0413. So the adsorption capacity for bilirubin is the most excellent. When initial bilirubin concentration is 300mg/L, the adsorption capacity is 1.95mg/ml gel in human plasma. (2) The denser the functional groups, the higher the adsorption capacity. With the increase of functional chains' length, the adsorption capacity strengthens. (3) The adsorbents based on Sepharose CL-4B or cellulose on which the butylamine is coupled can adsorb free bilirubin. It results in the separation of albumin bound bilirubin. (4) The adsorption capacity increases obviously with the rise of initial bilirubin concentration. The adsorption environments with higher temperature and lower ionic strength are advantage for adsorption. The equilibrium of adsorption can be reached within 0.5 hours. (5) The patients' plasma is dynamically adsorbed for 2.5 hours using adsorbent based on Sepharose CL-4B as support and coupled the butylamine as terminal functional group, the adsorption percentages of total bilirubin, direct bilirubin and indirect bilirubin are 55.2%, 57.1% and 52.2%, respectively. The adsorption capacity is more obvious than that of domestic resin adsorbents. (6) The adsorption capacity of adsorbent based on cellulose as support and coupled the butylamine as terminal functional group is less remarkable than that of adsorbent based on Sepharose CL-4B as support. The adsorption percentage of total bilirubin is only 13.2%; however, it is excellent that its nonspecific adsorption is less.