The Mechanisms Of Hyperhomocysteinemia Inducing Vascular Inflammatory Reaction And Inducing Atherosclerosis

Objective: To explore the state of hyperhomocysteinemia induces vascular inflammatory reaction in aorta of rabbit and observe the changes of cellular adhesion molecules (VCAM-1, ICAM-1), nuclear factor-κB (NF-κB p65),and thrombus precursor protein(TpP) in the process of artherosclerosis (AS),further to reveal the mechanism of the form of artherosclerosis (AS) by hyperhomocysteinemia . It could help people to learn the reason of AS and to provide a new therapy target.Methods: Sixteen rabbits were randomly divided into control group and experimental group. Two groups rabbits were collected 2.7ml anticoagulated blood before experiment respectively. Rabbits of experimental group were injected methionine [80mg/(kg.d)] subcutaneous every day for eight weeks, in order to establish the model of rabbit hyperhomocysteinemia. The rabbits of control group was injected isotonic Na chloride. After eight weeks, 2.7ml anticoagulated blood was collected and the rabbits were killed. the level of homocysteine(Hcy) and thrombus precursor protein(TpP) in plasma in the two groups were measured by enzyme linked immunosorbent assay(ELISA).The histopathological changes of aortic lesion were observed by light microscope .The expression and activation of nuclear factor-κB (NF-κB p65), cellular adhesion molecules (VCAM-1, ICAM-1) in endothelial cell in the two groups were investigated by immunohistochemistry.Results:1. The level of Hcy in blood plasma of experimental group rabbits were higher significantly than control group at the end of eight weeks[(45.02±8.04)μ mol/L vs (16.85±1.64) μ mol/L,P<0.05].2. Immunohistochemistry manifests :The positive rate of VCAM-1 were...