The Study About Virus Antigen Allocation And The Virus Proliferation Morphology Of Rabies Fixed Virus AG Train Within PHKC

The experiment makes use of the modern immune enzyme organize chemistry and the electricity mirror technology to carry out the system experiment. The experiment emphasizes the contrast test between the immunity fluorescence and immune enzyme organization chemistry(ABC) that all belong immune organization chemistry technology. We compare the two results that optics mirror and electricity mirror .There is most important meaning to prevent, diagnose and treat the rabies that master the biology process after the virus infection cell. in addition, the basic theory data have guidance significance to improve productive technics, rise output and enhance quality. Furthermore, that will afford to production and quality department the sensitivity, differential, simple and speed method of examine rabies virus antigen. Main research method: (1) ABC. According to the make peace by marriage and plain affine high high immunology peculiarity nature principle with biotin reaction,manufacture affine plain- biotin- hyperoxide enzyme compound reagent first. Ding-dang and biotin-rization two resists to contact time , whose affine not saturated vegetable may combine with second upper antibody biotins combining with location and then connects antigen-antibody reaction- system and ABC mark system into an integral whole, be used for the purpose checking. (2) immune fluorescence law. Principle being tied in wedlock according to the immunology antigen- antibody peculiarity, the antigen gets reaction again than corresponding , the compound and belt forming the antigen antibody have the certain fluorescein with the known number antibody molecule mark fluorescein. Make use of the fluorescence microscope , location and quantity observing fluorescence location strong or weak to ascertain an antigen within the cell. (3) electricities mirror be defeated dye a specimen. Use metal atom inside the heavy metal dye liquor to be electron "pigment" , lower density living things specimen (virus) parcel is wound but is form obvious contrast method. Thereby, that the electron light beam can pass the low electron density virus pellet but can not pass metal background, makes the virus pellet display obvious limpid structure , is to shoulder contrast.The result studying is indicated: ABC applying closes the cell chemistry and the indirect immune fluorescence technology in person , the poisonous antigen carrying out hydrophobia fixes position , demonstrate the poisonous antigen of hydrophobia fixes position in cytoplasm, the poison explaining hydrophobia breeds within the cytoplasm. Pass an electricity mirror process and virus particle form happened in the virus observing visible aG plant in cell inner form , hydrophobia is poisoned breeding within cytomembrane system , have amounts much viruses to breed especially within thick face endoplasm net cavity. Demonstrate existence also grows the free mature virus particle that abscission releases within cell gap from cytomembrane bud, these are to be difficult to ascertain that under glossy mirror. Also demonstrate the aG plant virus breeds within the cell not being confined to specially appointed film system.The result studying besides is indicated: The result basic the mirror the glossy mirror is observed bearing fruit with the electricity is observed is about that aG proliferates development in PHKC, consistent. Confirm the virus infection 72 hs queens equally, the infected cell begins to increase by , prolong with training time's, the cell inner virus breeds amounts increasing to 120 144 hs also gradually, the glossy mirror goes down the distinctive immune enzyme masculine gender cell and distinctive fluorescence cell rapid increase , the most cell assumes strong positive reaction. Electricity mirror observation has the amounts many virus buds life in cytomembrane system , endoplasm net pool inner accumulation has more virus particle , the cell gap also sees a few virus particles. Particle of cell gap virus particle quantity far inferior to that the cell combines virus amounts are many but. This result first step reveal gain virus liquid time last 120th with virus infection PHKC 144 is suitable. Virus amounts are not many according to the cell outside , are to improve virus gains rates but try to use cell disintegration , release out the virus particle being tied in wedlock within the cell , carry out a purification, appropriate improve a yield rate.