Rabies Vaccine And The Extract From Toad Skin Combined Effect Of Preventing Rabies

Objective : the Extract from Toad Skin has well enhances the organism immunity the function. Through the new depuration method, reduces may cause the side effect ingredient, with rabies vaccine union application ,prevention rabies .as a major rabies vaccine for the treatment of post-exposure immunization vaccines, immunization and produce high titer antibody and cellular immune good to play a crucial role in the prevention of rabies. In this paper, Vero rabies vaccine combined with the Extract from Toad Skin with high purity and small side effect measured in animal experiments of immunity.Methods : The Vero cell rabies vaccine experiments with the Extract from Toad Skin combined with a separate application for the control of rabies vaccine, immunization experiments, animals. 1. To observe the cellular immune level, we have adopted a rabies vaccine and the Extract from Toad Skin combined 0, 7 days intraperitoneal injection of BALB mice (rabies vaccine 0.5ml, the Extract from Toad Skin 10μg/ml), while another simple application of rabies vaccine group and the control group, 15 days after the mice were killed doing the following experiment : [1] spleen lymphocyte proliferation and transformation experiments[2]murine T lymphocyte surface markers Flow Detection : CD4+, CD8+ Detection and CD4+ /CD8+. The same time, we used macrophages phagocytic rate lymphocyte proliferation rate and macrophages phagocytic index to evaluate Cell Immunity Level using rabies vaccine and the Extract from Toad Skin the Joint application of 0, 7days neck subcutaneous injection of guinea pig (rabies vaccine with 0.5ml, the Extract from Toad Skin 10μg/ml), while set up another purely using the rabies vaccine group and blank control group, in paragraph 9 days abdominally admission macrophages measured phagocytic rate and phagocytic index To explore the Extract from Toad Skin application programs whether or not to rabies Vaccine Results in Significant have an impact in measured macrophages phagocytic rate lymphocyteproliferation rate the experiment we increase in the 0, 1, 2 days of the Extract from Toad Skin 0, 7 days been vaccinated against rabies a set of experimental. 2. In order to determine its humoral immune effect we were set simple application of rabies vaccine group and the Extract from Toad Skin combined group. 0, 3, 7, 14, 28 days intraperitoneal injection of BALB mice (rabies vaccine 0.5ml, the Extract from Toad Skin 10μg/ml) way to 4, 6, 13, 22, 29 days and the mice orbital collected from serum Vern detection at different times in serum rabies virus neutralizing antibody titer to assess the humoral immune levels and changes.3. In order to observe different mouse the Extract from Toad Skin application procedures for the protective effect, We separately establish the national rabies vaccine standard comparison group, the pure application rabies vaccine group and the Extract from Toad Skin union application group, and simultaneously determines attacks the poisonous quantity, performs the vaccine potency experiment:Group: 1 25 times of, 125 time of, 625 time of, 3,125 time of dilutions national rabies vaccine standard distinction abdominal cavities inject 0.5 ml/, 0, 7 days immunities.2 5 times, 25 times of, 125 time of, 625 time of dilutions rabies vaccine abdominal cavities inject 0.5 ml/ only for 1 hour latter 0, 7, the day immunity.3 5 times, 25 times of, 125 time of, 625 time of dilutions rabies vaccine abdominal cavities inject 0.5 ml/, after the immunity 1 hour distinguish the abdominal cavity to inject 10 u g/ ml the Extract from Toad Skin 1mlAfter 14 days simultaneously attack all groups, 28 days determinations. Calculates ED50 and the effect price. Compared with the pure application rabies vaccine group and with the Extract from Toad Skin union application group's effect price height, may take to the mouse the protection effect determination basis.The results : 1.In cell immunity : rabies vaccine and the Extract from ToadSkin combined group spleen cell proliferation rate, CD4 +, CD4+ /CD8 +and macrophage phagocytosis and phagocytic index significantly higher than the rate of application of rabies vaccine group. 2. In humoral immunity : rabies vaccine in combination with the Extract from Toad Skin the sixth after the initial immune response in mice can significantly improve the specificity of the rabies virus neutralizing antibody levels. 3. Protection of mice rate : rabies vaccine combined with the Extract from Toad Skin protection group was significantly higher than other groups, early application of enhanced the Extract from Toad Skin is no rabies vaccine protection rate, while the rabies vaccination procedure Application of the Extract from Toad Skin group immune best results. 4. The crowd rabies vaccine in combination with the Extract from Toad Skin neutralizing antibody markedly improved after the seventh day of the positive rates.Conclusion : the Extract from Toad Skin early mouse can be used to improve the level of serum antibody; To raise the level of immune cells; Early in particular to improve the protection rate protection; The early and the crowd can enhance antibody positive rates. The results of this combination provides a good theoretical basis for solving the simple application of rabies vaccine aluminum adjuvants can have in the early days of protective antibodies, by the Al-rabies vaccine adjuvant to non-rabies vaccine adjuvant for clinical transition period provides a practical immunization program, and for the future cytokines adjuvant replacement aluminum adjuvant provides a theoretical basis.