Pate Soft Tissue Damage To Brain Memory Influence Of Elder Mouse

The soft tissue damage usually is refer to soft tissue and part of cartilages and so on skin , subcutaneous, tissue, fascia, muscle, tendon, ligament, synovial membrane, ligamentum capoulare and the peripheral nerve, the blood vessel damage. May divide into the acute damage and the chronic damage according to the time. The human body soft tissue damage is in humanity kinematic scheme. One kind of common disease, frequently-occurring disease, Domestic, and foreign suffers from and this getting sick very many. Because the soft tissue damage may the acute damage and the chronicity accumulates the damage to cause to appear the neck to shoulder the legs and waist and the four limbs different situation, the varying degree symptom Some even induces many similar internal medicine department of neurology ,the gynecology department and so on the different symptom occurrence In the recent several dozens years, Observes, the research, he discovery study multi-illness very major part unceasingly after the medical arena colleague originates from the soft tissue muscle, the ligament, the fasia, the fat, the ligamentum, capoulare, the nerve, the blood vessel and so on sends directly sends, the damage very large. The native country inside and outside scholar to this sickness has made the comprehensive system exploration and the research work. In recent years ,Each place hospital starts the treatment which establishes the related soft tissue to damage to be transferred to another department, Causes the soft tissue to damage this discipline to approve in the medical treatment by more and more many colleagues and to acceptAfter pate soft tissue damage main clinical manifestation for headache, study and memory ability decrease ,The analysis the synthesis, the understanding. judgment ability drop obviously. This ex periment causes the cervical vertebar joint through the destruction mouse pate ligament method to have the gradual micro dislocation ,Creates arteria vertebralis mechanicalness bearing, Lacks the blood law using zhui-the basis artery Causes the brain to lack the anoxemia, the oxidized irritability damage The discussion pate soft tissue damages whether can cause the memory barrierObjective: If the instability of cervical vertebral column make the ability of study and memory decrease in mice. If there are the changes on pathology and histochemistry in model groups.Method:forty-four six-month old mice were randomized into two groups: control group and model group. The abdomen decubitus is fixed after the model group mouse ether anaesthesia, Pate disinfection, After neck center margin, Carries on the cascade separation with the artery forceps, Makes the double festival after the line to grip shears the neck myo-to tie, Exposed cervical vertebra ligament and 2nd cervical vertebra, Cuts between its spinous process and the cervical vertebra the ligment, Again uses diameter 1.0mm the electricity to congeal the needle cauterization to scald the side ligament, But after sutures the skin. Control group not after any processing. The change of learning and memory in mice were observed with step-down avoidance test, water-maze test and hole-board test. Pathological changes of brain tissue was examined by hematoxylin eosin (HE) staining, The activity of Super oxide dismutase (SOD), the content ofmaleic dialdehyde (MDA) in encephalon, and the content of nitric oxide (NO) in serum were investigated,The activity of lactate dehydrogenase(LDH) and Super oxide dismutase (SOD) were decreased,the content of maleic dialdehyde (MDA) in encephalon and the content of nitric oxide (NO) were increased. We observed the changes on the model groups vs. control group.Results:1 Ethology1.1 In step-down avoidance test: compared with the control ,the model groups indicated that the reaction period prolonged and wrong times increased ( P <0.01), and the latency period shortened significantly. There were no significance different changes between the two model groups (P>0.01). The results indicated that the learning and memory abilities of model groups decreased obviously.1.2 Water maze test :The whole-distance swimming time prolonged and mistakes increased in water-maze test(P<0.01). There was no significance different changes between the two model groups (P>0.01). The results indicated that the spatial discrimination ability of model groups was disordered seriously.1.3 In hole-board test, the latency period prolonged and the times of extending the holes decreased significantly(P<0.01). There was no significance different changes between the two model groups(P>0.01)2 Pathology2.1 HE straining of brain tissue: to observe the hippocampus and cerebral cortex, neuronal degeneration, cell necroses karyopycnosis, cell membrane unsharpness and so forth.2.2 Immunohistochemical staining withβ-amyloid (1-40): there are more buffy cell than the control in hippocampus and cerebral cortex.3. Biochemical indicator: The activity of Super oxide dismutase (SOD) was decreased, the content of malondialdehyde (MDA) in encephalon was increased, and the content of nitric oxide (NO) in serum was increased too, The activity of lactate4 dehydrogenase(LDH) was decreased.Conclusion:1. The model mice of cervical vertebra destabilization were set up by destroying around the cervical vertebra the ligament.2. The cervical vertebra destabilized mice that were induced by lactic acid can change the learning and memory abilities .3. The number of the hippocampus nerve cells were decreased by expressing strengthen oxygenize stress,Aβ,casepase-3 when The cervical vertebra destabilized.