Preliminary Study On The Effort That The Antibody Of Campylobacter Does To Nerve Ganglion Cell Of Colon

Objective: Neuronal intestinal malformation is one of the most common diseases to the pediatric surgeons including Hirschsprung's disease and allied disorders . Its pathological characteristics are the abnormity on quantity, quality and distribution of intestinal ganglion cell, which result in a series of clinical symptom such as constipation . The basic pathologic reason is absence of ganglion cells which is considered to result by environmental and genetic factors. These years more scholars consider environmental factors such as toxin and inflammation can damage the ganglion cell seriously. Now some scholars have found that the infection of cytomegalovirus can make the degeneration of ganglion cells. The study on the coloenteritis manifests that the infection of viruses is an important reason resulting in coloenteritis. We consider that the infection of viruses can result in the degeneration of ganglion cells not only by indirect ways like ischemia and hypoxia, but also by the direct ways like the injury of the toxin and antibody . Campylobacteria is considered the main reason of diarrhea in the world. The study indicates that diarrhea of children from 1 year old to 2 year old is mainly resulted by Campylobacteria. Campylobacteria results in pathologic change of ganglion cells such as Guillain-Barre syndrome by sticking, infestation, toxin and antibody etc. But can Campylobacteria result in the degeneration of ganglion cell of colon? and how does it result in the degeneration? We do not find those are reported in the publication. We observe the effect of the antibody of Campylobacteria and the ganglion cells both in normal colon and in the degenerate colon, then analyse the relation between the antibody of Campylobacteria and the generation of ganglion cells. Material and methods:[1] the samples of 30 patients with normal colon were collected .The patients age from 1 month to 4.5 years , average 2.23 ± 1.73 years old, and include 18 boys and 12 girls . All the patients without HD were clearly certified by barium enema, Anorectal manometry, and rectum mucosa biopsy. [2] The samples of 30 patients with HD were collected in the secondary hospital of SDU. Each sample was divided into four different parts: normal segment, dilatation segment, transitional segment and narrow segment. The patients age from 2 months to 6 years .average 3.53 ± 1.97 years old, and include 21 boys and 9 girls .All the patients were clearly diagnosed by barium enema, Anorectal manometry, and rectum mucosa biopsy.All the samples were fixed in 10% formalin, then were embedded in paraffin. Immunohistochemistry method was used to check the effect of the antibody of Campylobacteria to the normal and degenerative ganglion cells. SPSS11.0 software was used to deal with data. P<0. 05 has statistical significance. Results:(1)Brown—yellow granules can be found in ganglion cells of normal colon (about83.3%) .While there are a little brown —yellow granules in comparison group (about10%) .There is statistics difference between experimental group and comparison group.(2) There are brown—yellow granules in normal ganglion cells (about76. 7 %) and degenerative ganglion cells (about53.3%) .While there are a little brown — yellow granules in necrotic ganglion cells (about6.7%). There is statistics difference among normal ganglion cells, degenerative ganglion cells and necrotic ganglion cells. Conclusions: The antibody of Campylobacteria can combine with normal ganglion cells . There are some antibody of Campylobacteria combining with the degenerative ganglion cells .while there is a little antibody of campylobacteria combining with the necrotic ganglion cells.