| Objective:To approach endothelialization and long-term patency rate of ePTFE (expanded polytera fluoro ethylene) and Dacron blood vessel prostheses with bone marrow CD34+cells.Methods:CD34+cells were isolated from bone marrow by canine and purified by immunomagnetic beads system, Seeding the cells to ePTFE and Dacron prostheses which implanted abdominal aorta artery(AAA) and inferior vena cava(IVC). Dogs were separated into ePTFE experimental group and ePTFE control group and the Dacron experimental group and Dacron control group, Obtaining the specimens of the experimental groups and the control groups to compare the patency rate in the 10,30,60,100 days after transplantation; biopsy specimens, and immunohistochemistry method identified endothelial cell; gely observed by light microscopy and scanning electron microscopy, and at different times of endometrial hyperplasia and endometrial endothelial, Observation of neointimal thickness; more arterial and venous system after bone marrow CD34+vascular endothelial cells seeded situation.Results:(1)The isolated cells from canine bone marrow were CD34+cells by flow cytometry. CD34+cells which isolated and purified by immunomagnetic beads system, trypan blue staining living cell to cell counting 2.6(±0.3)×10~7/ml. (2)Identification of CD34+cells by immunohistochemistry of the vascular wall cells growing endothelial cell layer.(3)Implanted arterial occlusive vascular endothelial artificial rate of 0, 25% stenosis group ePTFE Polyester Unit restenosis rate was 25%. Group ePTFE graft implanted vein occlusion rate 12.5% 37.5%, restenosis rate was 28.5, Polyester Unit 0 restenosis rate was 37.5%. 0 implanted arterial occlusion rate control group, the control group implanted narrow vein occlusion rate of 100% to 100%.(4)HE staining was observed under light microscope polyester neointimal closely attached cavity surface intact. ePTFE neointimal HE staining in the face with ePTFE vascular among some separation. Endometrial thickness of two new experimental group than the control group graft neointimal thickness, Endometrial thickness of the experimental group was no significant difference between the two new vessels.(5)SEM:Since the experimental group and 30 days of artificial vascular anastomosis to the direction of the cavity surface intermediate artificial vascular endothelial cell density gradually reduce neointimal 174,900 days of artificial vascular endothelial cells of endometrial cavity surface-ranked uniform integrity. The new polyester vascular endothelial cell layer of endometrial and with a more complete, these cells were, Running consistent with the long axis of vascular cells. ePTFE vascular new endometrium compared with the corresponding period of polyester neointimal less complete, the lack of small cortical region, Cellulose and attaching to the surface of blood cells, during which see necrosis and senescence of endothelial cells. Intimal surface of the control group without artificial vascular endothelial cell coverage, the only fiber arrangement.Conclusion:(1)the separation of immunomagnetic beads system cells purified CD34+cells, proliferating ability, The number of cells can satisfy 30cm long, has a diameter of the cavity surface 6-8mm artificial vascular endothelial cultivation. (2) By immunohistochemical detection of the plant cells to vascular endothelial cell wall.(3) Purified CD34+cells were seeded on the ePTFE and polyester graft. have to get a good rate of endothelial and smooth.(4)Dacron grafts after implantation of CD34+cells and endothelial adhesion-cavity surface-level results were better than ePTFE Public vessels.(5)Artery after bone marrow CD34+vascular endothelial cells seeded patency rate than venous system and the degree of bone CD34 positive cells in pulp plant and the degree of vascular endothelial patency rate.
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