| Objective To investigate the impacts of Recombinant Human interferon alpha-2b (IFNa-2b) on the expression levels of (?)erum oxidative stress status in the treatment of chronic hepatitis B (CHB) with different genotypes.Methods Forty-seven patients with chronic hepatitis B and twenty-one healthy volunteers as a control were enrolled in this present study. In control and patients group, serum malondialdehyde (MDA) levels, serum total antioxidative stress capacity (TAC), serum glutathione (GSH) concentrations, glutathione S-transferases (GSH-ST), glutathione peroxidase (GSH-PX), glutathione reductase (GR), serum vitamin E (V_E)and vitamin C(V_c) were measured spectrophotometrically. After the therapy of interferon alpha-2b with the dose of 300 million units via intramuscular injection thrice a week for 12 weeks, these parameters were measured again in the patient group. The genotypes of hepatitis B virus were detected by Polymerase Chain Reaction and hybridization. The effective group was defined as the patients with complete response and partial response. Results1. The genotype B (21.3%) and genotype C (68.1%) take the most percentages of all the patients with HBV infection.2. There were 20 patients (44.4%) who have received complete response and partial response in all the patients receiving the IFNa-2b treatment. The significant differences of the effective rate in the groups with various genotypes and sexes have not been observed.3. The elevated concentrations of MDA and impaired levels of TAC in the patients with CHB were observed than those in the healthy controls (both P<0.05).There were no obvious differences in serum levels of MDA and TAC in CHB patients with various genotypes (P>0.05).The serum levels of MDA after the treatment with IFNa-2b were significantly decreased than the pretreatment levels (P＜0.05), which even returned to the normal concentration (P>0.05) in the effective group. The pretreatment levels of TAC were significantly elevated in effective group than those in the non-response group. There were obvious increases in the TAC after the IFNa-2b therapy in the effective group. However, the significant differences in the TAC levels before and after the INFa-2b treatment were no been observed in the non-response group.4. Obtained results indicated that the concentrations of glutathione, glutathione peroxidase and glutathione reductase of the patients were significantly lower than controls (all, P<0.05), while glutathione S-transferases was increased compared with healthy individuals (P<0.05). The concentration of ALT had a significantly negative correlation with GSH and GSH-PX, and positive correlation with GSH-ST. However, there was no significant correlation between the four indices and HBV DNA. The serum concentrations of glutathione and glutathione peroxidase were comparable in the patients with genotype C and those with genotype B.After three months of treatment of interferon alpha-2b,glutathione,glutathione reductase and glutathione peroxidase concentrations were significantly higher than the pretreatment levels (P<0.05).In the effective group, the pretreatment serum concentration of glutathione was significantly lower than that in the non-response group (P<0.05),while serum glutathione-S-transferase activity was significantly higher than that in non-responders (P<0.05).In the effective group after the INF therapy for three months, the serum concentrations of glutathione, glutathione reductase and glutathione peroxidase were increased significantly (P<0.05),which could be restored to the normal levels. In the non-response group after the treatment, the serum concentrations of glutathione reductase and glutathione was significantly lower than the healthy control group (P<0.05),and the concentration of glutathione peroxidase has been restored to the normal levels, while the activity of glutathione-S-transferase was still significantly higher than the healthy control group (P<0.05).5. The serum concentrations of vitamin C and vitamin E were significantly decreased in the patients with chronic hepatitis B than that in the healthy controls (P <0.05).The two indicators had no significant differences in the three HBV genotype groups (P> 0.05).Vitamin C and vitamin E concentrations were significantly negatively correlated with the level of ALT (r=-0.606,P<0.05;r=-0.602, P<0.05).However, there were no significant associations with HBV DNA (P> 0.05). After the treatment of IFN α -2b, the serum vitamin E concentration was significantly higher than the pretreatment level(P <0.05).Meanwhile, there was no significant increase in the serum vitamin C concentration in the non-response group. In the effective group the concentrations of vitamin C and vitamin E could be returned to the normal levels after the treatment.However, there was also a significant decrease in the vitamin E level in the non-response group than that in the health controls after the treatment.Conclusions There were the disturbances of oxidative status in the patients with chronic hepatitis B. It was also found that the oxidative stress be improved more or less in the IFN α -2b treatment of chronic hepatitis B patients. And we also suggest the theoretical basis of the anti-virus treatment with IFN α -2b in the course of the proper use of antioxidants in the chronic hepatitis B patients.
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