The Effects Of Hypoxia In Uterus On The Expression Of The Positive NNOS Cells In Parietal Cortex From Mice During Postnatal Development And The Ability Of Learning And Memory In Adult Mice

Objectives: 1. To study the effects of hypoxia in uterus on the expression of the positive nNOS cells in parietal cortex from mice during postnatal development (P1, P7, P14, P28, P90) and provide the theoretical reference for clinical neonatal hypoxic-ischemic encephalopathy. 2. To investigate the changes of the ability of learning and memory in adult mice after hypoxia in uterus and explore the effects of hypoxia in uterus on the ability of learning and memory in adult mice.Methods : 1. To use the model of hypoxia in uterus established by Tapanainen PJ and the mice during E13, E15, E17 cause hypoxia in uterus. The expression of the positive nNOS cells in parietal cortex from mice during postnatal development ( P1, P7, P14, P28, P90) with hypoxia in the inner of the uterus was studied with immunohistochemistry . 2. The ability of learning and memory in adult mice was observed by Morris water maze.Results: Compared with control group, The expression of the positive nNOS cells in parietal cortex from mice during postnatal development ( P1, P7, P14, P28, P90) in hypoxic group significantly decreased (P < 0.01), and latency to escape platform became more longer (1 d, 2 d, P < 0.05; 3 d, 4 d, P < 0.01), and the wearing numbers significantly reduced (P < 0.05).Conclusion : 1. hypoxia in the inner of the uterus could decrease the expression of the positive nNOS cells in parietal cortex from mice during postnatal development (P1, P7, P14, P28, P90). 2. Intrauterine hypoxia reduces the ability of learning and memory in adult mice.