A Research Of Using HPLC To Analyze And Half-make Chemistry Composition Of The Chinese Herbal Medicine

This text studied to use HPLC to analyze and half making chemistry composition ofChinese herbal medicine, the Chinese herbal medicine Codonopsis pilosula is example, inaddition to first step analyzing of it's chemistry composition, we also made to get monomer.The full text includes six experiments, test one is to evaluate the instrument function andoperating stability, the result enunciates the experiment condition can meet the demand; Testtwo is to elementary analyzing Codonopsis pilosula, the result explain the Chinese herbalmedicine Codonopsis pilosula still have many composition that tradition separation method ishard to get; Test three is using big bore resin to divide ethanol distilling Codonopsis pilosulainto three segments: 30%, 50%, 80%. Test four then is to analyze these three segments byHPLC; Test five is a few makings of compositions in segment 80% and then analysis thecompositions to evaluate their pure degree. Test six is to evaluate the amount of thecomposition from test five, the result indicates the amount of the xomposition can reach thelaboratory demand completely, After injecting 20-30 times, the amount of the composition canreach the experiment of cell medical efficacy.According to the analysis the result of each experiment, we can get: The traditionalseparation method result in many lapses of compositions during the period of step by stepseparating; This thesis adopt the big bore resin to divide ethanol distilling Codonopsis pilosulainto three segments segment, then we directly use HPLC to analyze and half makecompositions. This method can reduce the lapse of the compositions in the process ofseparation as far as possible, and we can also get pure composition.What this text investigate using HPLC to analyze and half make chemistry composition ofChinese herbal medicine is feasible, and it can provide a helpful method to explore the valid compositions of Chinese herbal medicine, it also provide a new thinking for us to investigatethe function mechanism of Chinese herbal medicine.