| Aging is an important risk factor in many diseases, such as Alzheimer's disease, Parkinson's disease and etc. Recent research has demonstrated that histomorphological changes in the aging brains have a near correlation with these diseases. We investigate the histomorphological changes in aging brain: the synaptic density, neuroglia changes and the expression of S100. Our goal is to understand the age-related changes happen in the brain.Materials and methods: 30 brains that died without clinical or pathological involvement of nervous system were obtained at autopsy. Sections from frontal lobe, occipital lobe, striatum and hippocampus were used for the SYNP, GFAP, Ferritin and S100 immunohistochemical staining. After observations of morphological changes of astrocyte and microglia, the density of SYNP was analyzed, and the S100IR cell was also counted. Differences of variables between the aged group and the control group were analyzed by Student's t-test.Results:1. The number of the GFAP positive astrocytes increased obviously in the aged brain.2. More active microglias appear in the aging brain that is the ferritin positive cells.3. The S100IR cells are mostly astrocytes, and the density of the S100IR cells increase in the aging brain.4. The synaptic's density decrease obviously in the aging brain, which appear a negative correlation with the rise of age.Conclusions:1. The astrocyte, microglia and the synaptic has an age-related change which may act as a protective effect for the brain. The density of synaptic decrease dramatically in human brain, and show a negative correlation with age.2. The increase of S100IR cell demonstrates that s100 play an important role for aging brain: stimulate the increase of astrocyte and microglia and protect the nerve cell.3. The expression of S100 has a positive correlation with astrocyte and microglia, but we could not find a near correlation between S100 and synaptic. |
PDF Full Text Request