| Objective To study the methods of bone defects repairing throngh bone marrow stromal cells induced by Osteoblast transplanted into bioceramic scaffolds in vivo.Methods Bone marrow stromal cells were obtained from the rabbits. The BMSCs were isolated , expanded , induced in vitro and co-cultured with osteoblast in the proportion of 2:1. The composites of osteoblast / BMSCs Implanted into the rabbits with artificial tibia bone defects after transplanted into bioceramic scaffolds at 1×106/ml,Implantation of BMSCs with bioceramic scaffolds alone was used as control. At 1 and 2 months after implantation , the specimens were harvested and the osteogenetic activity was evaluated by gross, X-ray and histological observation.Results:①Scan electric microscopy result: BMSCs were conglutinated and expanded on the surface of the Nano-bioceramic scaffolds completely within 24 hours.②On histological slices: obvious tubercular tissue were observed in the bone defects area of rabbit 1 month after implantion, a mass of fibre tissue, osteoid tissue and small quantity of bone sunken were observed through histology and after 2 months bone matrix and bone trabeculas were observed in new bone tissue, especially in experimental group.③On the X-ray film: 4 weeks later, the bone density between experimental group and control were not statistically significant (t=2.26.P>0.05);but after 8 weeks ,the bone density of experimental group and control was 147.00±14.79, higher than that of control 121.33±5.03 significantly(t=2.84,P<0.05).④Dynam-examination: 4 weeks later, compressive strength of new bone in experimental group and control were respectively 3.10±0.12 Mpa,2.47±0.18 Mpa, the difference was statistically significant(t= 2.26,P>0.05); 8 weeks later compressive strength of new bone of experimental group was 4.63±0.31 Mpa, higher that of the control 3.52±0.29 Mpa, (t=3.86,P<0.05).Conclusion Osteodifferentiation of MSCs induced by Osteoblast existed significantly in vivo.
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