| OBJECTIVES: To investigate the inhibitory effects of Genistein(GEN) on mouse allergic contact dermatitis(ACD);To investigate the relationship between the inhibitory effects of GEN on mouse ACD and the balance of type 1 and type 2 cytokines through detecting the levels of type 1 or type 2 cytokines produced by draining lymph node cells and offering experimental basis for the clinical application of GEN on the disease caused by type IV allergic reaction such as ACD. METHODS: The animal model of ACD was induced by DNFB, the effects of different doses of GEN(8, 20, 80mg/kg) on mouse ear swelling, histopathological changes of mouse ear skin, thymus index , spleen index , body weight and the toxic reaction were observed .The levels of type 1 cytokines (IFN-γ, IL-2, TNF-α) and type 2 cytokines(IL-4, IL-10) in supernatant of cultured draining lymph node lymphocytes in mice were measured with LuminexTM 100.RESULTS: All groups of GEN inhibited mouse ear swelling induced by DNFB significantly, reduced the edema of epidermis or dermis, inhibited the infiltration of inflammatory cells, decreased thymus index (P <0.05), the middle or low dose of GEN increased spleen index (P <0.05), high dose of GEN decreased spleen index(P <0.05); GEN did not affect the increase of mouse body weight(P <0.05), had no obvious toxic reaction. GEN inhibited the up-regulating of IFN-γ, IL-2, TNF-α , IL-4, IL-10 in supernatant of cultured draining lymph node lymphocytes in sensitized mice significantly(P <0.05).CONCLUSIONS: GEN can inhibit mouse ACD induced by DNFB significantly; GEN affect the relative weight of thymus and spleen significantly. GEN can inhibit the up-regulating of type 1 cytokines (IFN-γ, IL-2, TNF- α ) and type 2 cytokines (IL-4, IL-10) in supernatant of cultured draining lymph node lymphocytes in sensitized mice significantly, which might be one of the mechanisms that GEN inhibit mouse ACD. GEN doesn't affect the increase of the mouse body weight and has no... |
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