The Effect Of Sanshengyin On The Expression Of Caspase-3 And NNOS Protein In The Rats After Focal Cerebral Ischemia

Background Stroke is the third-leading cause of death in China. Currently, tissue plasminogen activator (tPA) is the only approved treatment used to reduced the disability of patients with acute ischemic stroke, but this compound has a very narrow therapeutic window of 3 h and the extent of its protective effects are limited. Evidence suggests that apoptosis cell death contributes to the progression of tissue damage following cerebral ischemia. Protective effects of traditional Chinese medicines have been widely reported. San Shengyin decoction is reported to be Calcium antagonist after stroke. To investigate the effect of SanShengYin in the rats after focal cerebral ischemia is helpful to study herbs ischemia antagonist mechanism.Objective: To investigate the effect of SanShengYin on the expression of caspase-3 and nNOS protein in the rats after focal cerebral ischemia. Methods: The model of focal cerebral ischemia and reperfusion was induced. The Sprage-Dawley rats were dividedrandomly into four groups: normal group,ischemia group, SanShengYin treatment group, Nimodipine treatment group. mmunohistochemistry method analyze the expression of caspase-3 and nNOS protein positive cells at 6h,12h,24h after repedusion.Compared the results between the group of normal,MCA0, Sanshengyin and Nimodipine.Results:Compared with the results of the MCAO group, caspase-3 and nNOS protein positive cells of Sanshengyin group and Nimodipine group were reduced obviously(p<0.05). Sanshengyin group is significant higher than Nimodipine group (p<0.05). Conclusions: SanShengYin and Nimodipine can protect nerve cell by reducing the expression of caspase-3 and nNOS protein,when the cerebral ischemia reperfusion injury occurring.