The Experimental Research Of Animal Model Treatment Of Bone Granulosus

Objective Through the establishment of the bone Echinococcus granulosus animal model and the experimental study of the treatment, explore the infection mechanism, the patho-change, the imageology feature and the effectiveness evaluation of the treatment. Method Take out the daughter cyst under asepsis condition from the liver of the sheep which infects the echinococcosis under natural condition, cut to pieces, remove the cyst skin, flush with 0.9% asepsis physiological saline and precipitate three times, make the HE dyeing counting, make the suspension which is 1200 scolex/0.1ml, then vaccinate directly nearby spinal column, under the hind leg periosteum and in the medullary cavity of the leg bone of the health grown-up meriones meridianus to prepare the animal infection model, then carry on the medicine and the surgery treatment to the model. Result The protoscoleces of bone Echinococcus granulosus direct inoculation may prepare the typical animal infection model. Use the medicine to treat for three months, we can find the medicine ingredient ABZSN in all of the experimental animal's blood, but in the cyst, the medicine density is extremely low. The pathological slices of the experimental animal's liver and kidney has no exceptionally response. The bone hydatid cyst continues to grow, the cyst surface is smooth, the color is clear, the shape is normal. Through the pathological slices observation, the germinal layer and the laminated layer of hydatid cyst do not have the change, but the laminated layer occasionally has the calcification. After the surgery treatment, observes for 3 month, no hydatid cyst recrudescence. Conclusion Although the experimental animal's oral administration dosage of the Liposomal Aibendazole is 10...