| Object To study the effect of adherent endotoxin of alumina ceramic wear debris on secretion of TNF-α cytokines by human monocytes in different time and concentration, searching after the role of endtoxin in artifical joint biological loosening. Methods Alumina ceramic wear debris were produced in vitro (mean sizes: <10μm)and the wear debris were heated by 250℃ for 3 hours .The monocytes were drew from health people blood. The 24 holes culture dish were divided randomly into 3 groups. Group A (8 holes) alumina ceramic particles free endotoxin were co-cultured with the monocytes ascontrol; while adherent Endotoxin of concentration (1EU/ml) alumina ceramic particles were co-cultured with the monocytes in group B(8 holes), adherent endotoxin of concentration (5EU/ml) alumina ceramic particles were co-cultured with the monocytes in group C(8 holes). Alumina ceramic wear debris adherent endotoxin with different concentration were co-cultured with the monocytes (debris volume (μm~3) to cell number ratios of 100:1). The production of TNF-α were assessed by enzyme-linked immunoabsorbent assay at 2, 6, 12 16, 24, 48-hour after co-cultured repecting.Results When the monocytes were exposed to adherent endotoxin of different concentration to alumina ceramic wear debris, the levels of TNF-α in group B , C were more than the control group . The production of TNF-a were significant difference both 6-hour's in group B and the 2, 6, 12,hour's in group C(P<0.05). Conclusion The method of drying alumina ceramic wear debris heat on... |
