Establishment And Primary Application Of A Mouse Model With Hepatitis B Virus Replication

Part oneHydrodynamics-based in vivo transfection results in high level expression of HBsAg in mouse liverObjectiveTo investigate whether an efficient expression of exogenous DNA in liver can be achieved by intravenous injection of DNA using a hydrodynamics-based in vivo transfection method, so as to provide a methodological base for molecular biological studies of hepadnavirus.MethodsMice were rapidly (in 5-8 seconds) injected with a large volume of pVAX1/S plasmid DNA solution via tail vein(the volume of DNA solution is equivalent to 8% of the body mass of the mouse, e.g. 1.6ml for a mouse of 20g), compared with the control mice which were injected slowly.The...