Study On Antibiotic Resistance Of Escherichia Coli In Healthy Human And Mechanisam Correlated Of Resistance Gene Dissemination

ObjectiveTo investigate the level and characterization of antibiotic resistance in Escherichia coli strain isolates from healthy human of Beijing.Detect the presence of integrons and gene cassrttes in multiresistant Escherichia coli isolates. To find out the relationship between gene cassettes and resistance patterns, analyze the distribution of integrons among different genotype isolates.And to illuminate the molecular mechanism of initegrons and gene cassettes in the development of miltidrug resistance and dissemination of antibiotic resistance. MthodsEscherichia coli isolates from healthy human were indentified by Vitek32, susceptibility to 14 antibiotics and ESBLs detection, susceptibility to another 7 antimicrobial agents determined by the agar diffusion method. Compare the identify of results between two antibiotics susceptibility assays. The frequency of class I and II integrons in isolates were detected by PCR. The amplicons of class I integrons with identical size was analysis by RFLP and purified to DNA sequencing. Find out the content and order of gene cassettes in integrons...