The Changes In The Expression Of Versican And CD44 Of Rat With Hemisection Of Spinal Cord Injury

Objective: To investigate the expression changes of Versican ofCSPGs family and its ligand CD44 in spinal cord in 3d, 7d and 14d following hemisection of spinal cord injury (hSCI). Furthermore, to explore the relationships of Versican,CD44 and spinal cord injury. Besides, to offer the morphological datum for the research concerning the spinal cord repair.Method: 20 adult healthy Sprague-Dawley rats were randomlydivided into sham-operated control group and day3, day7, day14 spinal cord injury groups. The spinal cord of every rat was taken out from the rostral and caudal segments of lesioned site. Then the tissue blocks were made into 25μm frozen sections and the immunohistochemistry ABC method was performed on these sections. We respectively observe and count the number of Versican and CD44 positive cells in ventral horn of spinal cord for all groups, and the average OD (optical density) value of immunoreactant were detected with computer image analysis technique.