Study On Different Purification Process And Blood Enrichment Effect Of Si-wu-tang

Background of resesrch: SWT(Decoction of four ingrendients)is composed of Radix rehmanniae,Radix Angelicae sinensis,Radix paeoniaea abla and Rhizoma chuanxiong.It is a famous traditional Chinese medical formula for enriching and regulating the blood.The former prescription is medicinal broth,and now there is a granule.Pharmacopeia of china record it as a mixture,all dosage form have problems of crude , large , black and so on.The prblems are exist in praeparatum of traditional Chinese drug. As a result ,the dosage to take is large, curative effect is unstability ,It is unconvenient to conserve and transport.Objective: To retain active component and get rid of impurity. Not only to reduce the does of the prescription effectively , but also to keep it convenient and to be appied to idustry.Methods: First, in the puifying process of the si-wu-tang, three technologies were used, such as ethanol precipitation, adsorption clarification, macroporous reisin .The evaluation idex included the weight of extraction,reservation rate of paeoniflorin and ferulic acid.Second,in the Pharmacodynamic experiment,the evaluation idex of blood enrichment effects included the total number of RBC, HGB, HCT , WBC and PLT.the blood deficient mice model which is induced by injecting APH and CPA is applyed to appraise the different puifying technologies.Results Both of ethanol precipitation, adsorption clarification can retain the active components of paeoniflorin and ferulic acid, but they donot reduce the weight of extraction. Macroporous reisin not only retain the active components of paeoniflorin and ferulic acid, but also reduce the weight of extraction.In the pharmacodynamic experiment of blood enrichment effects,the result show that The techinique of Alcohol-precipitation can promote the level of HGB, RBC, HCT, WBC in...