Effect Of Bovine Colostrum On Lymphocyte Of Mice

Objectives: In recent years, the people have conducted the massive research regarding immunoloregulation effect of bovine colostrums, but the most research technique was simple, the confidence level was not strong. Using the modern immunology experiment technology and the equipment carry on in-depth, careful, objective research to confirm immunoloregulation effect of bovine colostrum, which will certainly has the vital significance to health foods development of bovine colostrum, down to the entire dairying development. This experiment unified flow cytometry, the monoclonal antibody technology, the cell fluorescence chemistry technology and unilized the union principle of antigen and antibody to examine T lymphocyte subgroup, the B lymphocyte change in circumference blood of the mice through the flow cytometry; At the same time, the agarose gelatin electrophoresis examined bovine colostrum effect on programmed cell death of thymus. Thus, appraised immunoloregulation effect of bovine colostrum at two aspect of immune cell quantity and programmed cell death of immune organMethods: This experiment took 40 KunMing mice, stochastically divided into 4 groups, each group of 10. According to 1, 10, 20 times of equivalent dosage on human recommendation quantity(2g/The date /60Kg) supposed low dose group, medium dose group, high dose group and control group. Low, medium, and high dose group fed bovine colostrums. At the same time, control group fed isometric the physiological saline, pouring continuously stomach 30 days. After end time pouring-stomach has been 24 hours, each group stochastically took 8 mices and took the circumference blood from the eyeball; Processed sample used the flow cytometry to examine. Main examination target included assistant nature T lymphocyte(Th), suppression nature T lymphocyte(Ts), B lymphocyte. Moreover the experiment examine increasing heavy, the chest gland index and the spleen index of the mice in 30 day-long.At the same time, this experiment took 40 KunMing mice again, stochastically divided into 5 groups, each group of 8. According to 1, 10, 20 times of equivalent dosage on human recommendation quantity(2g/The date /60Kg) supposed low dose group, medium dose group, high dose group, negative control group and positive control group. Low, medium, and high dose group fed bovine colostrums. At the same time, control group fed isometric physiology saline, pouring continuously stomach 15 days. After end time pouring-stomach has been 12 hours, each group stochastically took 5 mices and every mice was injected dexamethasone 25mg/kg except negative control group in the abdominal cavity. Mice was executed and took thymus after 16h. At one time, the agarose gelatin electrophoresis examined programmed cell death.Results: (1) Each group's difference isn't remarkable about Th lymphocyte quantity (P> 0. 05); Each group's difference isn' t also remarkable about Ts lymphocyte quantity (P>0. 05); The...