Studies On The Secondary Metabolites Of The Three Marine Microorganisms

Studied on the chemical constituents of the fermentation and mycelia of three strains by all kinds of chromatographical techniques such as silica gel chromatography, Sephadex LH-20, PTLC and HPLC etc. 16 Compounds were isolated from the three strains and determined by means of physicochemical evidences and spectral methods. 5α , 8α-Epidioxyergosta-6, 22-dien-3β-ol(Sa) , 9(Z)-octadecenoic acid monoglyceryl ester(Sb) , ergosta-8 (9), 22-diene-3β, 5α, 6β 7α-tetraol(Sc) , ergosta-7, 22-diene-3β, 5α, 6β-triol(Sd) , pentitol (Se) , carbamide(Sf) , uracil(Sg) were isolated from Rhizopus sp.. 3-Isopropyl- pyrrolopiperazine-2,5-dione (Ta), 5α, 8α-epidioxyergosta-6, 22-dien-3β-ol (Tb) , pyrrolopiperazine-2,5-dione (Tc) , thymine (Td) , uracil(Te), mannitol(Tf) were separated from Oceanospirllum sp.. From metabolites of Micromonospora sp., dibutylphthalate(Fa) , phthalic acid bis-(2-ethyl-hexyl)ester(Fb), ergosta-5,7,22-triene-3 β-ol(Fc) , thymine (Fd) , Kojic acid(Fe) , amber acid(Ff) were obtained.Among them, compound Sc,Fa, Fb were isolated from their genus for the first time.