Determination Of Active Constitutents In The Branches And Leaves Of Taxus Yunnannensis

The chemical constituents contained in the Traditional Chinese Medicine (TCM) is very complicated. The material foundation of the TCM taking efect is the cooperation of various substances, Previously, taxanes have been extracted from yaw tree by liquid solvent extraction. The large quantities of organic, and often chlorinated, solvents used are, however, both expensive and environmentally unfriendly. Many studies have been conducted to overcome such problems and to provide alternate routes for obtaining taxol and its related analogues. In this thesis, a supercritical fluid extraction based separation process has been developed for this purpose .but the traditional quality evaluation to the TCM, depending on the quality or quantity of some or several chemical constituents, is dificult to evaluate availably the true good or bad of it, (especially in the complex prescription preparation). HPLC has many merits such as higher sensitivity, higher eficiency, and broader scope.The scientific name of Taxus is Taxus chinensis in 《Chinese Plants》 ,Plant classify for gymnosperm subphylum , taxus eye, taxus chinensis subject, taxus chinensis belongs , It belongs to 23 kinds that red legume family includes 5, Our country has 4 kinds and a mutation, Respectively, Taxus wallichiann Zucc, Taxus yunnannensis, Taxus chinensis, Taxus cuspidat。 Widespread, but the quantity is rare, comparatively scattering , seldom slowly grow into forest and grow. Yunnan Province of our country Taxus chinensis main distribution area of resource, and Taxus yunnannensis no matter the number of trees or storage capacity account for more than...