The Study Of Dendritic Cells Culture And Killing Effect By T-Lymphocytes After Activiated By Dendritic Cells Loaded With RCC 786-0 Cell Line Freze Thawing Antigen In Vitro

Objective :To study the methods of induction,biological character of dendritic cells(DCs) which derived from the peripheral blood of health adult,and further to study the anti-tumor of kidney effect activated by DCs in vitro.It will provide the experimental foundation for clinical Renal cell carcinoma immunotherapy of DCs.Methods :1.The induction and biological character of DCs derived from human monocytes Monocytes isolated from the peripheral blood of health adult are cultured in medium.compare to the cultural effect with different cell factors and bolting optimal cultural method.at 37℃in humidified 5% CO2 in air in medium with rhGM-CSF,rhIL-4 and TNF-αfor 7-10 days, DCs from human monocytes are analyzed on morphology, cellar structure under optics and electronic microscope,Stronger allo-MLR is one of DCs characteristics,it is performed by using MTT assay to observe the ability of DCs stimulating lymphocytes proliferation.and then...