Expression And Significance Of TGF-α And PTEN In Cervical Squamous Carcinoma

Objective:To study the expression and its significance,and correlation of TGF-α and PTEN in cervical squamous carcinoma and cervical intraepithelial neoplasia(CIN).Methods:Immunohistochemical staining TGF-α and mutated PTEN were performed by the standard Elivision technique for the paraffin-embeded sections of 82 cervical squamous carcinomas and 40 CINs.The relationship between expression of TGF-α and PTEN with the clinicopathologic features was analyzed, respectively.Results:1. The TGF-α protein expression was mainly found in cytoplasm. The positive rate in carcinoma , CIN and chronic cervicitis were 59.76 %( 49/82), 20 %( 8/40) and 15 %(3/20)respectively; the positive rate of TGF-α was higher than those in CIN and chronic cervicitis(P<0.01) , and was not associated with pathological grading(G1-G3),clinical staging ( I -III),age and lymph-node metastases(P>0.05).2. The positive rate of mutated PTEN protein in carcinoma,CIN and chronic cervicitics were 63.41%(52/82 ), 35 %( 14/40) and 30%( 6/20 Respectively. The difference of the expression of PTEN protein between the cervical squamous carcinoma and the chronic cervicitis or CIN was significant (P < 0.01).PTEN had closely relative to clinical staging (P<0.05) and lymph-node metastases(P < 0.01),but not to pathological grading and age(P>0.05).