The Protective Study Of Aldose Reductase Inhibitor On Corneal Tissue Of Early Diabetic Rats

OBJECTIVE: To observe the effect of aldose reductase inhibitor(ARI) on the activity of corneal aldose reductase (AR) and Na+-K+ATPase , and on corneal lesions in early diabetic rats and to investigate the protective mechanism.METHODS: Healthy adult male SD rats were randomly divided into normal control group, diabetic group and diabetic group treated with Baicalin or Fidarestat. Diabetic rats were induced by one time injection of streptozotocin(STZ) intraperitoneally. The period of experiment are twelve weeks. The activity of AR and Na+-K+ATPase in the cornea of all rats were quantified at the sixth week and the twelfth week. The histopathologies of cornea in each group were observed by Light microscopy and Transmission electron microscopy at the twelfth week. RESULTS:1 Compared with normal control group, the corneal AR activity of the diabetic group, the Baicalin treated group and the Fidarestat treated group was significantly increased, and it increased gradually during the period of experiment. Moreover, the increased amplitude of the corneal AR activity in the diabetic group was markedly higher than that of the two treated groups; Compared with the diabetic group, the corneal AR activity in the two treated groups was apparently decreased at each experiment point; There was no difference between tow treated groups.2 Compared with normal control group, the corneal Na+-K+ATPase activity of the diabetic group, the Baicalin treated group and the Fidarestat treated group was significantly decreased, and it improved apparently in the Fidarestat treated group at the end of the experiment; Compared with the diabetic group, the corneal Na+-K+ATPase activity in the two treated groups was apparently increased at each experiment point; Fidarestat manifested better effects than Baicalin.