Interleukin-1α-induced Gene Expression In Normal Ovarian Surface Epithelial Cells And Ovarian Cancer Cells

Objective: The majority of ovarian cancers (85%～90%) are believed to derive from the ovarian surface epithelium which is composed of a single layer of flat-to-cuboidal epithelial cells. Ovulation is believed to contribute to the development of ovarian cancers that derive from the ovarian surface epithelium(OSE). The process of ovulation is synonymous withinflammation and inflammatory cytokines such as interleukin-1α (IL-1α ) have recently been shown to induce both inflammatory and anti-inflammatory responses in human OSE (HOSE) cells. In this study we directly compared levels of IL-1α -induced gene expression by analyzing the levels of 11β -hydroxysteroid dehydrogenase(11β HSD) types(11β HSD-1) and 2 (11β HSD-2), cydooxygenase-2(COX-2) , IL-1 receptor (IL-1R) and glucocorticoid receptor α (GRα)mRNA between normal HOSE cells and ovarian cancer cell lines derived from poorly differentiated (SKOV-3, BG-1, PEO-4) and well differentiated (PEO-14)ovarian adenocarcinoma to investigate the mechanism of ovarian cancer.Methods: Normal HOSE cells and ovarian cancer cell lines were cultured in vitro. The basal levels of 110 -hydroxysteroid dehydrogenase(11β HSD) typesl(11β HSD-1) and 2(11β HSD-2), cydooxygenase-...