The Clinical Study Of HLA-B*27 Subtype And Ankylosing Spondylitis

OBJECTIVE: To explore the role of HLA-B*2704, 05 gene subtype in the development of ankylosing spondylitis (AS) and the correlations between HLA-B*27 gene subtype and clinical manifestations, serological characteristics, diagnosis of patients with AS in the Han Nationality of Shandong area population.METHODS: In 83 AS patients and 90 matched healthy control subjects, HLA-B*27 were detected by using the methods of flow cytometer(FCM). Polymerase chain reaction-restricted frag- ment length polymorphism(PCR-RFLP) were used to get the gene frequencies of HLA-B*2704,05. Rheumatoid factor (RF) was detected by latex agglutination. C-reactive protein (CRP) was determined by immunoturbidimetry.RESULTS: Compared with controls, an increased gene frequency of HLA-B*27 was found in FCM (RR=14. 30, X~2=132. 2, P < 0. 05) and PCR-RFLP (RR=14.27, X~2=133.4, P < 0.05). Both methods showed no significant differernce (P>0. 05).There was no significant difference between the HLA-B*27~+ and HLA-B*27~- patients with respect to sex (X~2=0. 0021, P>0. 05), sacroiliac joint lesion (14/79 VS 0/4, P> 0.05) and extra-articular manifestations (22/79 VS 0/4, P>0. 05) such as urethritis, enteritis and uveitis. But the disease was more severe in males than that in females. Only two subtypes were detected- HLA-B*2704 and HLA-B*2705. No significant differences were found in the distribution of the different subtypes...