The Mechanism Of DHPO Protecting Against Chronic Aluminum-Exposed Mice

Objective:Aluminum (Al) is ubiquitous in the environment, and is extensively used in contemporary life.Food additive, drugs and utensils comprising of aluminum are main sources of aluminum uptake in China. Aluminum is a known chronic neurotoxicant. There is evidence that aluminum has been linked to neurodegenerative disorders including Alzheimer's Diseases (AD), dialysis encephalopathy (DE). In present, it can be affirmed that aluminium is the only factor associated with DE. Employees in aluminum refinery could obviously be caused mental dysfunction. Chelators have been useful to treat aluminium-overload diseases in clinical practice. In the following experiments , DHPO(1,2-dimethyl-3-hydroxy-4-pyridone) is used as a new type of aluminum chelating agent in animals .We investigate the potential of DHPO to improve the learning and memory ability and to change the expression of apoptosis-related protein Bcl-2 and Bax in hippocampus, aimed to evaluate the protect of DHPO against aluminum-exposed mice .At the same time, we would observe if DHPO can reduce aluminum concentration in the animals and cause the removal or displacement of other essential metals to provide foundation for seeking a safe and efficacious chelator for aluminum overload.Methods1. The synthesis of DHPODHPO is synthesized from 2-methyl-3-hydroxy-4-pyrones and Methylamine (CH₃NH₂) via heating at 95～100℃6.5h .2. The effects of DHPO on learning and memory impairment caused by aluminum in mice...