The Study Of Parkin And Pink1 Gene In Patients With Parkinson's Disease

Objective1. To explore the mutation of the gene(parkin PINK1) in PD patients by PCR and DNA sequencing.2. To explore the relationships between polymorphisms in exon 7 and 9 of Parkin gene and PD.3. To investigate the PINK1 gene mutation between the EOP and LOP. Methods1. 40 consecutive PD patients were recruited in this study.. And these patients were from Qilu Hospital. The diagnosis of PD was made according to the UK PD Brain Bank criteria. Individuals with atypical parkinsonism or taking sedatives were excluded. Patients were interviewed in the clinic by a movement disorders physician regarding demographics, disease characteristics, medications and whether they had a sleep problem.2. 40 PD patients were assessed with the following battery of tests: Hoehn & Yahr stage (H/Y); Questionnaire of Non-motor Complications in Patients with Parkinson's Disease; Unified Parkinson's Disease Rating Scale (for 40Chinese PD patients).3. 40 Chinese PD patients and 40 normal controls had genetic test. Exons 5 7 9 11 12of parkin gene and exons 2-4 of PINK1 gene were amplified by PCR. PCR products were observed using Agarose Gel Electrophoresis. Among them exon 5 of parkin gene and exons 2,3 of PINK1 gene were sequenced.For exon7 and 9 of parkin PCR products were digested by restriction enzyme and observed using PAGE. All 40 PD patients were assessed by MMSE and Unified Parkinson's Disease Rating Scale (UPDRS).