Detection Of Aberrant Methylation Of RASSF1A Gene In The Tissue And The Serum From Ovarian Tumor Patients And Its Clinical Significance

Objective: To detect the aberrant methylation of Ras association domain family 1A (RASSF1A) in the tissue and the serum from ovarian tumor patients; to explore the relationship between the aberrant methylation of RASSFl A gene in the serum and in the tissue of ovarian tumor and ovarian cancer, and to analyse the association of the aberrant methylation of RASSFl A gene with some clinical factors, including FIGO(Federation International Gyneclogy Organization,FIGO) stage ,histologic cell types, degree of cell differentiation and lymph node metastasis.Methods: Preoperative peripheral blood of 35 cases of ovarian cancer patients and matched cancer tissue specimens were obtained from 2004.12 to 2005.09 as the experiment group; 30 cases of benign ovarian disease tissues and matched serum and 10 cases of normal healthy serum spiecimens were collected as the contrast group. 8 cases of 7 days' postoperative serum specimens were collected and contrasted with Preoperative serum spiecimens. Each sample was detected by methylation-specific polymerase chain reaction (MSP).Results: 1.The aberrant methylation rate of RASSF1A gene in ovarian cancer tissues was 31.4%(11/35), and was zero in benign ovarian disease tissues (0/30). There was significant difference between them (p<0.05). 2.The aberrant methylation rate of RASSF1A gene in ovarian cancer serum was 20%(7/35), and none of benign ovarian disease serum and normal healthy serum was methylated. There was...