| ObjectiveTo investigate the relationship between the hepatocyte apoptosis, TNF-a and oxygen free radical in alcoholic liver disease (ALD) in rats. MethodsThe dose of 40% ethanol (8g/kg. d,) was administered intragastrically by gavage twice daily in ALD model rats for 8 weeks , the control rats was received isovolume saline by gavage. End of 8th week, the activities of serum ALT and AST were detected by autobiochemistry instrument, the TNF-a levels of serum was determined by radio immunoassay method, and maleic dialdehyde (MDA) was determined by thibabituric acid(TBA) quantifying method. Meanwhile, the pathological changes of liver was observed under light microscope after HE staining, and hepatocyte apoptosis was detected by the TUNEL method.Results1. Compared with that in the control group, the level of serum ALT and AST were significantly increased (107.4±16.6 vs 48. 4±10. 5IU/L, 232. 1±52.9 vs 102.9 ± 14. 1IU/L, (P <0. 05) , the content of TNF-a and MDA were significantly increased (1236. 4 + 283. 5 vs 745. 6+174. 8ng/L; 41.53 ± 7.43 vs 15. 72 ± 2. 06nmol/ml (all P (0.05) in the ALD group2. Compared with that in the control group, the hepatocytes became swelling and macro- and microvesicular stratosis , spotty and focal necrosis, and inflammation were observed in model group. The hepatocyte apoptosis were significantly increased{P (0.05) and the TUNEL positive cells were located around the central vein, spotty and focal necrosis in the ALD group..
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