Study On Effects And Mechanism Of Checking Tumour Of The Extract Of The Bi Ginkgo Loba

S180 cells was used to identify the functions of the bi Ginkgo :.oba extract. The extracts were prepared from the bi Ginkgo loba by the way of ethanol soaking, low pressure distill, organic reagent abstraction. Finally, the desired part of Juglans mandshurica maxim was got by means of chromatography. Primary experiments showed that LD50 and 95% range is 640. 12-395. 38mg/kg respectively.In the inhibited experiments on proliferation of cells, S180 cells were innoculate to the cavity of nice. During the logarithm growth phase, extracts from Juglans mandshurica maxim with various doses were added. 5-Fu was used to be positive controls and cultured medium used to be blanking controls. MTT method was used to detected 0D values. Inhibited ratio was calculated E.nd curve was drew.Serological pharmacological tests were carried out. Intragastric 2 times for sequential 3d. These mice were fasted diet for 12h while no water banned. The blood were taken after 1 h administration, then was inactived under 56 for 30 min. The serum with drug was treated by steriled methods. The various doses were given to mice by intragastric administration. With the same dose to the mice, various extracts were added to be various dosage groups. MTT method was used to detected 0D values to identify the optimized diluted solution.Some immune index such as lymphocyte transformation function, NK cells activities, TNF activities and IL-2 induced level were determined.Results: Within the range of 4. 125-33. OOug/ml, the extract of Juglans mandshurica maxim show remarkable inhibited function on proliferation of S180 cells. From the inhibited curve, it also demonstrates that this function is dose-dependence.