Genotyping Hepatitis C Virus From Yixing Country Jiangsu Province And Study On The Variation Of The Second Envelope Glycoprotein E2 In These HCV Strains

HCV is an enveloped, positive-strand RNA virus belonging to the Flaviviridae family. Around 180 millions persons in the world are thought to be infected with HCV and 3-4 millions persons will be infected with it per year. Hepatitis C, it had become clear that the vast majority of persons who develop acute hepatitis C-perhaps over 80 percent-remain infected. A high proportion of persons with chronic HCV infection will advance in time to cirrhosis and progress eventually to terminal chronic liver disease, in some instances after developing HCC. Hepatitis C is a growing public health concern. So, it is urgent to find out the factor which responsible for chronic HC so that we can take effective prevention measures. For this reason, we carry out a population-based molecular epidemiological study on HC from etiology angle in Yixing county of Jiangsu Province.Part I Study on hepatitis C virus genotyping in Yixing county Jiangsu provinceHCV is characterized by a high mutation rate of its RNA genome. This genetic heterogeneity leads to six genotypes and a number of subtypes. It is generally accepted that the different prevalence of HCV genotypes is linked to geographical area. Genotype has been identified as a useful predictor of responses to treatment and is correlated with the severity of HC prognosis. As the basic data for a systematic investigation study it is essential to learn about the genotype distribution in this area. We identification the genotypes by the Rt-PCR with type specific primer designed according to the sequence of 5 ' non-coding region (5' NCR)...