Protective Effects Of Edaravone On Hyperoxic Acute Lung Injury In Neonatal Rats

Objective To explore the effects and mechanisms of Edaravone on hyperoxic acute lung injury in neonatal rats so as to provide experimental and theoretical evidences for preventing and treating hyperoxic lung injury in neonate. Methods A total of 96 3-day-aged Sprague-Dawley(S-D) rats were randomly divided into 4 groups :Ⅰair + normal saline;Ⅱair + Edaravone;Ⅲhyperoxia + normal saline;Ⅳhyperoxia + Edaravone . GroupⅢand groupⅣwere exposed to 90%～95% oxygen, while the other two were exposed to atmosphere . GroupⅡand groupⅣunderwent intraperitoneal injection of Edaravone in dose of 5mg/kg per 12 hours while other two received the same dose of normal saline. At the end of exposure(in third day and seventh day), total protein(TP) , malondialdehyde (MDA ) , superoxide dismutase (SOD) and TNF-αin bronchoalveolar lavage fluid (BALF ) , the lung wet weight/dry weight ratio (W/D) were determined and the intension of nuclear factor -κB(NF -κB) expression in rat lung was analyzed by the method of immunohistochemistry,and lung histopathological changes were examined in all groups.Results (1) After 3 days, compared with groupⅠ, groupⅢshowed acute lung injury characterized by the increase in the concentration of TP , MDA , TNF-αin BALF and the intension of NF -κB in rat lung while the decrease in the activity of SOD in BALF (P <0.01) and presence of congestion, edema, inflammatory infiltration. After 7 days, compared with groupⅠ, besides the continuous increase in the concentration of TP , MDA , TNF-αin BALF and the intension of NF -κB in rat lung and the continuous decrease in the activity of SOD in BALF (P <0.01), W/D also increased in groupⅢ(P <0.01) ,and lung histopathological presence showed more severe changes in groupⅢ.(2) After 3 days or 7 days ,compared...