| Human GL50, a newly identified member of B7 superfamily, is a type I membrane protein of about 45-72kD encoding 309 aa.GL50, cloned by Swalow M.M. in 1999, is originally identified as human B7h located at 21q22.3 with the length of 3.4kb, which is mainly expressed on B cells, macrophages and some organs as heart, skeletal muscles, testis and lung. The interaction of GL50 with ICOS, the specific receptor for GL50, is critically involved in the activation, proliferation, differentiation and cytokine production of T cells as well as in the antibody secretion from B cells during the secondary immune responses. It is well-accepted that GL50/ICOS signal pathway plays a pivotal role in the regulation of inflammatory responses, in the prevention of transplantation rejection and tumor development, as well as in the modulation of autoimmune diseases. Therefore, the modulation of ICOS/GL50 signal pathway, not only provides a new perspective for a thorough understanding of the delicate regulation of the immune system, but also plays a potential role in clinical application.This study was based on preparation of ICOS transfectants and mouse anti-human GL50 monoclonal antibody 11C4 to investigate the GL50 biological function in the regulation of immune responses.1. The expression of GL50 on activated T cells.After T cells being activated by PHA or by anti-CD3 with anti-CD28 mAbs, the GL50 and ICOS expressions on activated T cells were detected by means of FACS on Oh, 24h, 48h, 72h, and 96h. The results showed that GL50 and ICOS expressions were up-regulated on activated T cells. Furthermore, the results were verified through the... |
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