| Cell rheology is a branch of comprehensive trans-subjects studies involving many subjects such as macro-rheology, molecular rheology, cell biophysics, cell biochemistry and its research methods refer to analytical chemistry, image processing, computer processing, electronics, materials science as well as advanced testing technologies. Therefore, the development of this subject will be continuous in future.Above all, this dissertation shows a kind of cell deformability analysis system developed by ourselves which is based on MEMS and microfluidic technology. Comparing with normal rheological detection technology, our methods show their beneficial on many ways, such as abundant information quantity, flexible and controllable structure, easy to operate and cheap.The emphasis of my dissertation is the laboratorial application studies based on our analysis system, including evaluate and improvement of the system, and broaden the application fields, especially making a particular discussion on the rheological and biological characteristics of drug-loaded intact human erythrocytes. From the results of our experiments, we found there were different graded changes in drug-loaded intact human erythrocytes, such as deformability reduction, membrane damage, morphologic abnormality, and higher brittleness. These changes can directly affect the elimination time and releasing effects of drug-loaded intact human erythrocytes in vivo. In author's opinion, rheological and biological characteristics should be recongnized and will become a important index in clinical reaserch.The primary tests of this system show that data and results are almost identical with the object of research. And we also drew a conclusion that this new method is an economical, convenient, accurate, multi-information and effective method on cell rheology research as well as clinics. The study also makes the foundation for the further development of the new method. |
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