The Expression Of Tumor Associated Antigen MAGE-1 And It's Function Of Anti-tumor

Background and objectiveThe MAGE gene family is composed of 23 related genes divided into four clusters located in regions q28(MAGE-A genes), p21.3(MAGE-B genes), q26(MAGE-C genes), and pll(MAGE-D genes) of the X chromosome. The MAGE-A subfamily comprise 12 genes(MAGE-l to MAGE-12) that 60% ~ 98% identity in their coding sequence. The MAGE-1 gene is located in the end of Xq28 and is composed of three exons. The DNA of MAGE-1 is 4.5kb long, while the mRNA of it is 1722bp long. Recently, there are many evidences to show that MAGE-1 gene highly expressed in various type of tumors but silent in normal tissue, inflammation tissue and carcinoid. Up to now, eight MAGE-1 epitopes recognized by cytolytic T Lymphocytes had been found. MAGE-1 antigen is particular interest for antitumor immunotherapy because it is strictly tumor specific and shared by many tumors. The lack of expression of encoding genes in healthy tissues should be ensure strict tumoral specificity of immune response obtained after vaccination of patients with this antigen.The purposes of this study were to develop a MAGE-1 gene vaccine and to observe its effects of anti-tumor in vivo and vitro.