Plasmids Construction Of Dengue Ⅱ Virus Envelope Glycoprotein For Eukaryon And Procaryon Expression

Background and objectiveDengue virus (DV) is a member of mosquito-borne flaviviruses and possesses a positive-stranded RNA genome. And DV has 4 distinct serotypes (DV-1, 2, 3, 4). All of them can result in classical dengue fever (DF) and/or dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). Every year there are about 100 million DF patients and 500 000 DHF/DSS patients in the tropics and subtropics worldwide. Now WHO already put DHF/DSS, Hepatitis, Malaria and Tuberculosis into the list of the most four serious infectious disease.however, the pathogenesis of DV infection is not clear , and there the is not an efficient way to deal with the diaease in the clinic therapy .. Epidemics of DHF/DSS have more frenquent since the 1980s in the tropics and subtropics worldwide such as Southeast Asia and South PRC. Therefore, DV infection has become the severe problem of the public health.There are the main three theories about the pathogenesis of DHF/DSS: (1) antibody dependent enhancement (ADE) infection hypothesis: DV specific IgG was thought to induce the immune disorder; (2) the factor of virus itself: the virulence or replication ability of virus is the main reason of the serious disease; (3)immuno-pathogenesis theory: the cross-reactive T...