Expression Of L1CAM Protein And MRNA In The Gut Of The Congenital Hirschsprung's Disease

Background and objectiveHirschsprung's disease (HD) is a congenital malformation of the embryonic hindgut characterised by the absence of parasympathetic intrinsic ganglion cells in the submucosal and myenteric plexuses. It is regarded as the consequence of the premature arrest of the craniocaudal migration of vagal neural crest cells and is therefore regarded as a neurocristopathy. L1 cell adhesion molecule (L1CAM) is realized that expressed widely in neural system and compact with the migration of the intestine neuron. Recently mutations of L1CAM have been identified in patients with HD. But it only is in few individual case. By the methods of RT-PCR and immunohistochemistry, the expression of L1CAM gene and protein was detected in the large intestine of HD and corresponding normal tissues. It was observed that the relation between expression of L1CAM (mRNA and protein) and pathologic characteristics and the positive correlation between mRNA and protein in the HD . In addition, the paper presumed the functions of L1CAM in the etiopathogenisis of HD.Materials and methods1.The expressions of L1CAM protein was observed in 48 cases of surgically...