| Objective: The prevalence of obesity and diabetes mellitus has increased continually, and the research of its aetiological role has made deep finding. Free fatty acids (FFA) exert an important role in the occurrence of obesity and insulin resistance. Free fatty acids are important metabolic substrates for the pancreatic–cell, and long term exposure of pancreatic islets to elevated concentration of free fatty acids results in an alteration of fasting blood glucose. The detrimental effects of a long–term high–fat diet (HFD) on the pancreatic–cell function and can lead to type 2 diabetes mellitus (T2DM), and this is so–called lipotoxity. Obesity is a risk factor of type 2 diabetes mellitus. Its occurrence attributes to energy metabolism disequilibrium. My experiment aimed to observe the changes of fasting blood glucose and serum free fatty acids composition. This study was to provide basis revealing the relationship between nutrients and diabetes and to provide a useful animal model of type 2 diabetes mellitus for wide experimental study.Methods: 48 healthy Wistar male rats (8 weeks old) were divided into two groups at random, 12 in control group and 36 in high–fat group. They were assigned to the following treatment for 28 weeks, the control group was fed with normal food and the high–fat group was fed with high–fat food. Every four weeks measured the body weight of rats. From the tenth week, every six weeks measured the fasting blood glucose using blood...
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