| Background and objective:.Early and effective closure of an open wound is a critical point at the early stage of severe burns, when the autologous skin is insufficient. The burn surgeon is in a dilemma of alloskin transplantation because of the conflict between the rejection to allografts and the potential side-effects of immunosuppressive drugs, i.e. infection. Therefore, it is crucial to induce allospecific tolerance to donor. The liver plays a critical role in inducing immunological tolerance, because it's interposition of between the gastrointestinal tract and the general circulation. This specific immunologic tolerance induced by intragastric or portal venous (p.v.) route has been described as "Hepatic tolerance" or "Portal venous tolerance" . The p.v. injection, however, requires abdominal operation and has the potential risk such as bleeding, infection, portal vein thrombus, etc.It is reported that neuraminidase (Neu)-treated hematopoietic cells lose terminal sialic acid residues of ascialoglycoprotein on their surface and exhibit an enhanced affinity for liver. This selective removal is mediated through an interaction with a liver receptor which is specific for galactose-terminal glycoprotein molecules. This study is aimed to select for a appropriate concentration of Neu that enhances the migration of Neu-treated donor bone marrow cells (dBMCs) to the liver, and investigate the effect of Neu-treated dBMCs on hematopoietic foci formation in internal organs and chimerism in peripheral blood, and observe the survival of skin allografts in the treatment of short-term cyclosporine A(CsA) application combined with intravenous injection (i.v.) of Neu-treated dBMCs in rats.Materials and methods:1. The BMCs from male SD rats were treated with four concentrations(0, 0.5, 1, 2 U/ml) of Neu (New England BioLabs Inc.) at 37° for 30 min, labeled by 99Tcm and injected via the tail vein of female Wistar rats in four groups, respectively. After five hours, the...
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