The Proliferation Of L02 Human Hepatocytes In Tolerized Genetically Immunocompetent Rats

An animal model especially rodent plays an important role in the development of hepatology. Hepatitis virus own species-specificity, susceptible hosts are confined to higher grade primate such as human, macaque and chimpanzee. Most of them are limited by various reasons.An animal model of rats with chimeric human liver is that human hepatocytes are transplanted into rat liver. It can be used to identify hepatitis virus just because the problem of species-specificity being resolved. With immunodeficiency or induced immunotolerance rats, it can establish animal model of rats with chimeric human liver infected with Hepatitis B virus. The animal model of tolerant rats with chimeric human liver can be applied in pathogenesis, antiviral therapy and development of vaccine.Human hepatocytes transplanted into rats just can survive a limited time. The live time of transplanted hepatocytes must be prolonged to establish a persistent animal model. With the exogenous growth stimulus such as partial hepatectomy, the recipient hepatocytes can not only regenerate, the transplanted hepatocytes also proliferate. If restraining the regeneration of recipient hepatocytes, following by the exogenous growth stimulus, the proliferation of transplanted hepatocytes can be improved significantly. We investigate whether human hepatocytes could repopulation after transplantation to rats with a normal immune system treated with 2-acetaminofluorene (2-AAF) or Retrorsine (Rts) and partial hepatectomy (PH).L02 Human hepatocytes were injected into the peritoneal cavities of fetal Sprague- Dawley rats to induce immune tolerance to L02 human hepatocytes. The 2 or 3-week rats were injected with 2-AAF or Rts. The L02 human hepatocytes stained with DiI were transplanted into the spleen via intrasplenic injection.. By various methods, we detected the proliferation of transplanted hepatocytes.