Identification Of Liver-cancer Adhere Peptide (LAP) And The Studies Of The LAP's Receptor Protein

China is a country of high incidency on liver cancer and more than 110,000 persons died from liver cancer every year which takes up 45% of all the liver cancer deaths in the world. At present, the main means on cancer therapy are surgical treatment, chemotherapy and radiotherapy,on which there are still lots of limitations and side effects.Recently,tumor targeting therapy has sprung up,that is making use of a highly special adhere-tumor stuff as a carrier to bring the medicine to the tumor only and stable the medicinal effects.The tumor's targeting therapy is becoming the most popular reseach topics on biology and medicine,that will be a new way to tumor therapy.Currently, monoclonal antibody as a major tumor medicine carrier has been accepted clinically.But most of the antibodies are generated from rats,so we have to acknowlege their heterology between rats and human-beings.The rejecting reaction would depress the pharmacodynamic action.We had better find a kind of non-antibody-like carrier which can target mainly tumor tissue but normal tissue.We have panned the targeting peptide to tumor for three times with phage-peptide display technique from the random phage peptide library and got several tumor-specific monoclones. Peptide, as a medicine carrier ,has low weight and strong affinity to the cells.In addition ,they can be cleared from blood and non-targeted tissue and penetrate the targeting cells stably.For obtaining the low weights and small peptide,phage shell should be got rid of from the phage-peptide and the left peptides are synthesized chemically. Tumor related protein or tumor signal protein may be researched through the liver-tumor adhere peptide as the detector.Their biochemical characteristics and integration mechanisms with the peptide may be useful for carrier's optimization.and the therapy of tumor.This paper shows the identification and localization of liver-cancer adhere peptide by immunohistochemistry or immunocytochemistry methods, and the researchment on the peptide's receptor by biochemistry and mass chromatography techniques.Bioinformatics software Swiss-modler is used to analyse the result.The experiment results show that the liver-cancer adhere peptide A54 can target to the liver tumor tissue or cells specially but normal liver tissue.Exactly it adheres to the membrane surface of the tumor cells from the immunoflucytochemistry...