Expression And Purification Of MgPa Recombinant Protein Of Mycoplasma Genitalium And Characterization Of Its Immunocompetence

Objective:In order to find a new antigen for the exploiting diagnosis of M. genitalium,a recombinant vector containing the gene encoding immuno-dominant epitope (1075¹364 amino acid residues )of MgPa attachment protein (MgPa' ) of Mycoplasma genitalium (M. genitalium) was constructed, the recombinant protein was expressed in E.coli Rosetta^TM2(DE3) and purified, and the immunoreactivity and immunogenicity of the recombinant protein was analyzed.Methods:The immuno-dominant epitope of MgPa gene from M. genitalium complete genome was chosen by computer analysis and then was amplified by polymerase chain reactions (PCR).The PCR product was subcloned into the expression vector pET-30a(+) to generate recombinant plasmid pET-30a(+)/MgPa', and MgPa' protein was expressed in E.coli Rosetta^TM2(DE3), and analyzed by SDS-PAGE and Western-Blot. The fusion protein was purified with Ni-NTA affinity chromatography, and the protein concentration was determined by the BCA protein assay kit. Zealand rabbits were immunized with the fusion protein and antibodies to MgPa' in sera were detected by indirected ELISA to evaluate the immunogenicity of rMgPa' . The immunoreactivity of the recombinan protein rMgPa' was analyzed by using the purified rMgPa' as the coating antigen in ELISA to test sera obtained from M. genitalium infected and normal human.