Apoptosis Induced By Resveratrol In Human Osteosarcoma MG63 Cells

Objective: Resveratrol (trans-3,4',5-trihydroxystilbene) is a common polyphenolic phytoalexin that is found in grape skin, peanuts, giant knotweed rhizome, and other food products. It ha- s been reported to have a variety of estrogenic, anti- inflammat- ory, anti-platelet, and anti-carcinogenic effects. Some reports have shown that resveratrol is a potential chemopreventive age- nt of cancer and it can prevent tumor growth and metastasis in human lung carcinoma, prostate cancer, pancreatic cancer, liver cancer, breast cancer, and leukemia models. Osteosarcoma is the most commonly malignant bone tumor with primary appearance during the second and third decade of life. At present the therapy of osteosarcoma still has much difficulty. It need to search some new methods. Therefore, this study was designed to investigate human osteosarcoma MG63 cells apoptosis induced by resveratrol and its molecular mechanisms. It may provid exp- erimental foundation for osteosarcoma.Methods: MG63 cells were cultured in the environment of 37°C,5%CO2 with modified Eagle medium (MEM) medium. The logarithmically growing MG63 cells were used. 1 The preparation of drug's solution: Stock solution of resveratrol was made in dimethylsulfoxide (DMSO, Sigma,...