Analysis On Genotype And Distribution Characterization Of HFRS Pathogen In Epidemic Areas Of Hebei Province

Objective: To understand the rat density, population constitution of rats, positive rate of host animals carried hantavirus (HV) and the distribution of genotype and subtype in Hebei epidemic areas. Analyze the nucleotide variation of HV, in order to guide the prevention and control for this disease.Methods:1 Investigate the epidemic areas; Capture rats and take rat lungs in sterile environment;2 Rat lungs were sliced with freezing microtome. Then HV positive lungs were screened with IFA after they were fixed by cold acetone;3 RNA of HV was extracted from positive lungs and cDNA was synthesized;4 RT-nested PCR was conducted to identify the genotype of HV by using primers of SEO type and HTN type, which were designed according to the standard strains of HV;5 PCR products of rat lungs, which came from different districts of Hebei province, were sequenced after purification. Phylogenetic trees were constructed according to G1 and G2 segments and distribution of gene subtype, genovariation and...