The Mechanism Of Ginsenosides Rg1 And Rb1 Promoting Neurotransmitters Release

Objective: To study the mechanism of ginsenosides Rg1 and Rb1 promoting neurotransmitters release. Ginsenosides Rg1 and Rb1 play a major role in modulating neurotransmission. Synapsins are phylogenetically conserved and highly abundant presynaptic phosphoproteins associated with the cytoplasmic side of synaptic vesicles. With the background, we therefore tested that ginsenosides Rg1 and Rb1 modulate neurotransmitter release in connection with synapsins phosphorylated by PKA.Methods: First, the amount of amino acids released from cell culture was measured by high performance liquid chromatography (HPLC). Briefly, the amounts released into the assay buffer were measured by HPLC with a fluorescence detector. The KRH buffer was collected without stimulation, that is, the amount of glutamate in the sample was considered as basal release. Sample fractions were collected every 1 min (sum total seven time points) into eppendorf tubes on ice by the batch method. Each group was joined to 10μM Rg1 or Rb1 at the second time point end. H89, a PKA inhibitor, was applied for 30 min at 10μM before collecting sample fractions. Second, PC12 cells were fixed in 4% paraformaldehyde containing 4% sucrose and 4 mM EGTA for 10 min at room temperature. After fixation,...